Tocrifluor T1117
Tocris Bioscience, part of Bio-Techne | Catalog # 2540
Fluorescent cannabinoid ligand; fluorescent form of AM 251 (Cat. No. 1117)
Key Product Details
Description
Fluorescent cannabinoid ligand; fluorescent form of AM 251 (Cat. No. 1117)
Product Description
Tocrifluor T1117 is a fluorescent form of AM 251. AM 251 conjugated with 5-carboxytetramethylrhodamine (5-TAMRA) that fluoresces at 543 nm excitation (590 nm emission). Displays GPR55 binding activity. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.View more information regarding Tocrifluor T1117.
Optical Data
| λabs | 543 nm |
| λem | 590 nm |
| Application | Fluorescence Microscopy, Confocal Microscopy |
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Product Specifications
Molecular Weight
974.97
Formula
C56H53Cl2N7O5
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N-(Piperidin-1-yl)-5-(4-(4-(3-(5-carboxamidotetramethylrhodaminyl)propyl))phenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide
CAS Number
1186195-59-4
PubChem ID
57369426
InChI Key
RNAPRXIZPJDYPH-UHFFFAOYSA-N
SMILES
CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)NCCCC1=CC=C(C=C1)C1=CC=C(C=C1)C1=C(C)C(=NN1C1=CC=C(Cl)C=C1Cl)C(=O)NN1CCCCC1)C1=C(O2)C=C(C=C1)N(C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.75 | 10 |
Preparing Stock Solutions
for Tocrifluor T1117
The following data is based on the product molecular weight 974.97
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 10.26 mL | 51.28 mL | 102.57 mL |
| 0.5 mM | 2.05 mL | 10.26 mL | 20.51 mL |
| 1 mM | 1.03 mL | 5.13 mL | 10.26 mL |
| 5 mM | 0.21 mL | 1.03 mL | 2.05 mL |
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Background References
References are publications that support the biological activity of the product.
- Bruno Beyond radio-displacement techniques for identification of CB1 ligands: the first application of a fluorescence-quenching assay. Sci.Rep. 2014 PMID: 24441508
- Daly Visualisation of vascular cannabinoid receptors and their potential interaction with α1-adrenergic receptors. Vascular and Smooth Muscle Phys.Soc. Symposium
- Sylantyev Cannabinoid- and lysophosphatidylinositol-sensitive receptor GPR55 boosts neurotransmitter release at central synapses. Proc.Natl.Acad.Sci.U.S.A. 2013 PMID: 23472002
- Daly Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteries. Br.J.Pharmacol. 2010 PMID: 20136833
Product Specific Notices for Tocrifluor T1117
For research use only
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