TBI

Name: TBI
Brand: Tocris Bioscience
SKU: 7660

Tocris Bioscience, part of Bio-Techne | Catalog # 7660

COA / SDS

Fluorogenic ligand for Broccoli RNA aptamer

Key Product Details

Description:	Fluorogenic ligand for Broccoli RNA aptamer
Chemical Name:	(Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	386.38

View Full Technical Details for TBI

Technical Data
Citations

Biological Activity for TBI

TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (K_D values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M^-1 cm^-1.

Licensing Information

Sold under license from Lucerna

Optical Data for TBI

Emission Color	Green
λ_abs	485 nm
λ_em	527 nm
Extinction Coefficient (ε)	35100 M^-1cm^-1
Quantum Yield (φ)	0.64
Application	Live-cell Imaging, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for TBI

M. Wt	386.38
Formula	C₁₈H₁₂F₂N₄O₂S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2863639-29-4
InChi Key	FFVXYOZVQLWDIF-NSIKDUERSA-N
Smiles	FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TBI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.73	20

Preparing Stock Solutions for TBI

The following data is based on the product molecular weight 386.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	12.94 mL	64.70 mL	129.41 mL
1 mM	2.59 mL	12.94 mL	25.88 mL
2 mM	1.29 mL	6.47 mL	12.94 mL
10 mM	0.26 mL	1.29 mL	2.59 mL

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Reconstitution Calculator

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References for TBI

References are publications that support the biological activity of the product.

Li Engineering fluorophore recycling in a fluorogenic RNA aptamer. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34490956

TBI