sBADA
Tocris Bioscience, part of Bio-Techne | Catalog # 7860
Green fluorescent D-amino acid for labeling peptidoglycans in bacteria
Key Product Details
Description
Green fluorescent D-amino acid for labeling peptidoglycans in bacteria
Product Description
sBADA is a green sulfonated BODIPY-FL 3-amino-D-alanine (sBADA) or fluorescent D-amino acid (FFDA). Labels peptidoglycans in live bacteria. Sulfonated form of BADA with increased hydrophilicity and thermostability. Excitation/emission λ ~490/510 nm.Optical Data
| Emission Color | Green |
| λabs | 490 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 49000 M-1cm-1 |
| Application | Fluorescence Microscopy |
Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
458.24
Formula
C17H21BF2N4O6S
Storage
Store at -20°C
Purity
≥94% (HPLC)
Chemical Name
(R)-2-Amino-3-(3-(5,5-difluoro-7,9-dimethyl-2-sulfo-5H-4λ4,5λ4-dipyrrolo[1,3,2]diazaborinin-3-yl)propanamido)propanoic acid
CAS Number
2376838-11-6
PubChem ID
138991774
InChI Key
OLMGTLBVHKBMSH-GFCCVEGCSA-N
SMILES
CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NC[C@@H](N)C(O)=O)=O)C(S(=O)(O)=O)=CC3=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.58 | 10 |
Preparing Stock Solutions
for sBADA
The following data is based on the product molecular weight 458.24
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 21.82 mL | 109.11 mL | 218.23 mL |
| 0.5 mM | 4.36 mL | 21.82 mL | 43.65 mL |
| 1 mM | 2.18 mL | 10.91 mL | 21.82 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.36 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665
Product Specific Notices for sBADA
For research use only
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