Rhobo6 ECM Dye
Tocris Bioscience, part of Bio-Techne | Catalog # 8876
Key Product Details
| Description: | Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues |
| Chemical Name: | (E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 756.26 |
Biological Activity for Rhobo6 ECM Dye
Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra. For optimal signal, we recommend using 561 nm excitation and a 575 nm long-pass emission filter. Rhobo6 staining is not compatible with samples that are chemically fixed or that otherwise exhibit compromised cellular membranes.
Used for: labeling the ECM in live tissues.
Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.
Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) measured in 2 M sorbitol to mimic binding, are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.
Please refer to this guide for further information on how to use this product.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Rhobo6 ECM Dye
| Emission Color | Orange |
|---|---|
| λabs | 535 nm |
| λem | 561 nm |
| Extinction Coefficient (ε) | 97,500 M-1cm-1 |
| Quantum Yield (φ) | 0.75 |
| Cell Permeable | No |
| Application | Fluorescent microscopy including 2P and STED microscopy |
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Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live kidney tissues. Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected kidney tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view. |
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Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live lung tissues. Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected lung tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view. |
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Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in liver kidney tissues. Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected liver tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view. |
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Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live small intestine tissues. Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected small intestine tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view. |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer