Rhobo6 ECM Dye
Tocris Bioscience, part of Bio-Techne | Catalog # 8876
Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues
Key Product Details
Description
Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues
Product Description
Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra. For optimal signal, we recommend using 561 nm excitation and a 575 nm long-pass emission filter. Rhobo6 staining is not compatible with samples that are chemically fixed or that otherwise exhibit compromised cellular membranes.Used for: labeling the ECM in live tissues.
Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.
Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) measured in 2 M sorbitol to mimic binding, are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.
Please refer to this protocol for further information on how to use this product.
Scientific Data Images for Rhobo6 ECM Dye
Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live kidney tissues.
Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected kidney tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view.
Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live lung tissues.
Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected lung tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view.
Use of Rhobo6 ECM Dye for labeling the extracellular matrix (ECM) in live liver tissues.
Rhobo6 ECM Dye was injected retro-orbitally into the bloodstream of a wild-type mouse, using the protocol described in the User Guide. Imaging was performed on live dissected liver tissues using single photon confocal microscopy, with 561 nm excitation and a 575 nm long-pass emission filter. Contrast is not normalized across fields of view.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Orange |
| λabs | 535 nm |
| λem | 561 nm |
| Extinction Coefficient (ε) | 97,500 M-1cm-1 |
| Quantum Yield (φ) | 0.75 |
| Cell Permeable | No |
| Application | Fluorescent microscopy including 2P and STED microscopy |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
756.26
Formula
C35H28B2N2O9.CF3CO2H
Storage
Store at -20°C
Purity
≥85% (HPLC)
Chemical Name
(E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate
InChI Key
RPHZIIQRPDDKIQ-OWCLOVKRSA-N
SMILES
FC(F)(C(O)=O)F.OB(O)C(C=CC=C1)=C1CNC(C=C2)=CC(O3)=C2C(C(C=C(C=C4)C(O)=O)=C4C([O-])=O)=C(C=C/5)C3=CC5=[NH+]/CC6=CC=CC=C6B(O)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.56 | 10 |
Preparing Stock Solutions
for Rhobo6 ECM Dye
The following data is based on the product molecular weight 756.26
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.22 mL | 66.11 mL | 132.23 mL |
| 0.5 mM | 2.64 mL | 13.22 mL | 26.45 mL |
| 1 mM | 1.32 mL | 6.61 mL | 13.22 mL |
| 5 mM | 0.26 mL | 1.32 mL | 2.64 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Fiore Live imaging of the extracellular matrix with a glycan-binding fluorophore. Nat.Methods 2025 PMID: 39915692
Product Specific Notices for Rhobo6 ECM Dye
For research use only
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