OG 488 BAPTA-1 AM
Tocris Bioscience, part of Bio-Techne | Catalog # 6256
Cell-permeable, fluorescent Ca2+ indicator
Key Product Details
Description
Cell-permeable, fluorescent Ca2+ indicator
Product Description
OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca2+ indicator (Kd =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.Optical Data
| Emission Color | Green |
| λabs | 494 nm |
| λem | 523 nm |
| Extinction Coefficient (ε) | 19000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescence Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
1258.06
Formula
C59H53F2N3O26
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
CAS Number
244167-57-5
PubChem ID
102028682
InChI Key
NQVWLDWXFNDFLA-UHFFFAOYSA-N
SMILES
FC1=CC2=C(C3=CC=C(C(NC4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=O)C=C3C([O-])=O)C6=CC(F)=C(OC(C)=O)C=C6[O+]=C2C=C1OC(C)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.26 | 1 |
Preparing Stock Solutions
for OG 488 BAPTA-1 AM
The following data is based on the product molecular weight 1258.06
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 79.49 mL | 397.44 mL | 794.87 mL |
| 0.05 mM | 15.90 mL | 79.49 mL | 158.97 mL |
| 0.1 mM | 7.95 mL | 39.74 mL | 79.49 mL |
| 0.5 mM | 1.59 mL | 7.95 mL | 15.90 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Thomas A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals Cell Calcium 2000 PMID: 11032777
Product Specific Notices for OG 488 BAPTA-1 AM
For research use only
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