NucleoLIVE™ Non-Toxic Dye
Tocris Bioscience, part of Bio-Techne | Catalog # 8935
Key Product Details
| Description: | Ready-to-use fluorescent nuclear stain for live cell imaging, cell toxicity, viability and morphology of 2D and 3D cell cultures |
| Purity: | ≥90% (HPLC) |
Biological Activity for NucleoLIVE™ Non-Toxic Dye
NucleoLIVE™ Non-Toxic Dye is a ready-to-use fluorescent nuclear stain for live cell imaging. NucleoLIVE™ Non-Toxic Dye is highly suited for long term 2D and 3D cell organoids imaging. Able to multiplex with Live-dead stain for multiplexed phenotypic imaging. NucleoLIVE™ Non-Toxic Dye is suitable for monitoring precise tracking of cell proliferation, differentiation, and responses to stimuli over time. Allow wash-free imaging. NucleoLIVE™ Non-Toxic Dye is provided as 50 µL of 1000x concentrate in DMSO. Excitation and emission maxima (λ) are 568 nM and 590 nM, respectively.
Please refer to this protocol for further information on how to use this product.
NucleoLIVE™ is a trademark of Saguaro Biosciences.
Licensing Information
Sold under license from Saguaro Biosciences
Optical Data for NucleoLIVE™ Non-Toxic Dye
| λabs | 568 nm |
|---|---|
| λem | 590 nm |
| Cell Permeable | Yes |
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NucleoLIVE™ Non-Toxic Dye Application. NucleoLIVE™ staining of 3D brain organoids under hypoxic conditions. Live imaging shows deep penetration and uniform nuclear staining (1:500, red), demonstrating suitability for complex 3D models. |
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NucleoLIVE™ Non-Toxic Dye Application. MCF-7 cells co-stained with NucleoLIVE™ (1:500) and Hoechst 33342 following 4-hour incubation. Image captured at 40× magnification, showing nuclear co-localization and live-cell compatibility. |
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NucleoLIVE™ Non-Toxic Dye Application. Comparison of proliferation rates in MCF-7 cells: stable nuclear GFP reporter line versus NucleoLIVE™ staining at various concentrations (1×: 1:1,000; 0.5×: 1:2,000; 0.25×: 1:4,000). Controls include untreated (“Medium”) and 0.1% DMSO. Assays performed on an Incucyte Live-Cell Analysis System by Prof. John Hawse, Mayo Clinic. |
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