NADA-green
Tocris Bioscience, part of Bio-Techne | Catalog # 6648
Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Key Product Details
Description
Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Product Description
NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.Please note that the spectral data is solvent dependant
Optical Data
| Emission Color | Green |
| λabs | 450 nm |
| λem | 555 nm |
| Extinction Coefficient (ε) | 25,000 M-1cm-1 |
| Application | Fluorescence Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
303.66
Formula
C9H9N5O5.HCl
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
CAS Number
2253733-11-6
PubChem ID
137919868
InChI Key
NKFVSANWNYYLLB-PGMHMLKASA-N
SMILES
N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.37 | 100 |
Preparing Stock Solutions
for NADA-green
The following data is based on the product molecular weight 303.66
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.29 mL | 16.47 mL | 32.93 mL |
| 5 mM | 0.66 mL | 3.29 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.29 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Kuru Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 2014 PMID: 25474031
- Ladokhin Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 2002 PMID: 12427023
- Kuru In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 2012 PMID: 23055266
Product Specific Notices for NADA-green
For research use only
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