MQAE
Tocris Bioscience, part of Bio-Techne | Catalog # 7856
Fluorescent Cl- indicator; membrane permeable
Key Product Details
Description
Fluorescent Cl- indicator; membrane permeable
Product Description
MQAE is a membrane-permeable intracellular chloride ion (Cl-) indicator. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. Excitation/emission maxima (λ) are 355 nm and 460 nm respectively. Can be used in fluorescence microscopy and flow cytometry, suitable for use in vivo and in vitro.Optical Data
| λabs | 355 nm |
| λem | 460 nm |
| Extinction Coefficient (ε) | 4850 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Insensitive |
| Application | Confocal imaging, Flow cytometry |
Spectra Viewer
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Product Specifications
Molecular Weight
326.19
Formula
C14H16BrNO3
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-(2-Ethoxy-2-oxoethyl)-6-methoxyquinolin-1-ium bromide
CAS Number
162558-52-3
PubChem ID
2762651
InChI Key
DSLLHVISNOIYHR-UHFFFAOYSA-M
SMILES
O=C(C[N+]1=CC=CC2=CC(OC)=CC=C21)OCC.[Br-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.26 | 10 | |
| DMSO | 3.26 | 10 |
Preparing Stock Solutions
for MQAE
The following data is based on the product molecular weight 326.19
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.66 mL | 153.28 mL | 306.57 mL |
| 0.5 mM | 6.13 mL | 30.66 mL | 61.31 mL |
| 1 mM | 3.07 mL | 15.33 mL | 30.66 mL |
| 5 mM | 0.61 mL | 3.07 mL | 6.13 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Wan An ATP-regulated ion transport nanosystem for homeostatic perturbation therapy and sensitizing photodynamic therapy by autophagy inhibition of tumors. ACS Cent.Sci. 2019 PMID: 30834321
- Verkman Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications. Anal.Biochem. 1989 PMID: 2751097
Product Specific Notices for MQAE
For research use only
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