JQ1-FITC
Tocris Bioscience, part of Bio-Techne | Catalog # 7722
Fluorescent BET bromodomain probe; suitable for use in TR-FRET
Key Product Details
Description
Fluorescent BET bromodomain probe; suitable for use in TR-FRET
Product Description
JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (KD,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.Optical Data
| Emission Color | Green |
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 7500 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Application | TR-FRET, Fluorescence polarization assays |
| Alternative For | FITC |
Spectra Viewer
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Product Specifications
Molecular Weight
1106.58
Formula
C50H50ClN7O9S2.CF3CO2H
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate
InChI Key
QRVVCZSOSIMDNN-LMDYQTDMSA-N
SMILES
O=C(NCCCOCCOCCOCCCNC(NC1=CC=C(C(C(O)=O)=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=S)C[C@@H]5N=C(C6=CC=C(Cl)C=C6)C7=C(SC(C)=C7C)N8C5=NN=C8C.OC(C(F)(F)F)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.07 | 10 |
Preparing Stock Solutions
for JQ1-FITC
The following data is based on the product molecular weight 1106.58
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.04 mL | 45.18 mL | 90.37 mL |
| 0.5 mM | 1.81 mL | 9.04 mL | 18.07 mL |
| 1 mM | 0.90 mL | 4.52 mL | 9.04 mL |
| 5 mM | 0.18 mL | 0.90 mL | 1.81 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Payne A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 2022 PMID: 35649410
Product Specific Notices for JQ1-FITC
For research use only
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