Janelia Fluor® 646 ADA New
Tocris Bioscience, part of Bio-Techne | Catalog # 8965
Red Janelia Fluor® fluorescent dye for live gram positive and negative bacteria imaging
Key Product Details
Description
Red Janelia Fluor® fluorescent dye for live gram positive and negative bacteria imaging
Product Description
Key information: Janelia Fluor® 646 ADA is a red fluorescent dye suitable for bacterial imaging applications, consisting of a bright Janelia Fluor® fluorophore bearing the unnatural amino acid D-Alanine. Incorporation of fluorescent D-amino acids in gram negative and positive bacteria can selectively label peptidoglycans in bacterial membranes, resulting in bright fluorescence around the bacteria and minimal or no signal detected inside.Used for: live gram positive and negative bacteria imaging.
Application: confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Correction Factor 280 | 0.19 |
| Application | confocal microscopy, flow cytometry |
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Product Specifications
Molecular Weight
582.73
Formula
C32H34N4O5Si
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(R)-4-((2-Amino-2-carboxyethyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
InChI Key
MNSAHQBYDUBJDU-AREMUKBSSA-N
SMILES
O=C(NC[C@@H](N)C(O)=O)C1=CC=C(C([O-])=O)C(C(C2=C([Si]3(C)C)C=C(N4CCC4)C=C2)=C5C3=C/C(C=C5)=[N+]6CCC\6)=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 5.83 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 646 ADA
The following data is based on the product molecular weight 582.73
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 17.16 mL | 85.80 mL | 171.61 mL |
| 0.5 mM | 3.43 mL | 17.16 mL | 34.32 mL |
| 1 mM | 1.72 mL | 8.58 mL | 17.16 mL |
| 5 mM | 0.34 mL | 1.72 mL | 3.43 mL |
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Product Specific Notices for Janelia Fluor® 646 ADA
For research use only
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