Janelia Fluor® 585, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 6418
Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
Key Product Details
Description
Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
Alternative Names
JF 585,NHS
Product Description
Key Information: Janelia Fluor® 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) < 0.0001. Exhibits low non-specific background staining.
Please see the protocol for further information on protein/antibody labeling and conjugation.
To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.
We also offer custom conjugation antibody services.
HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 585, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Orange |
| Brightness | |
| λabs | 585 nm |
| λem | 609 nm |
| Extinction Coefficient (ε) | 156,000 M-1cm-1 |
| Quantum Yield (φ) | 0.78 |
| Closest Laser line | 561 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.099 |
| Application | Confocal Microscopy, SRM, Two-photon Excitation Fluorescence Microscopy |
| Alternative For | Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594 |
Trademarks and registered trademarks are the property of their respective owners.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
649.60
Formula
C34H27F4N3O6
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
CAS Number
1811539-88-4
PubChem ID
132470913
InChI Key
GFNMIANIZWSVQR-UHFFFAOYSA-N
SMILES
O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 64.96 | 100 |
Preparing Stock Solutions
for Janelia Fluor® 585, NHS ester
The following data is based on the product molecular weight 649.60
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.54 mL | 7.70 mL | 15.39 mL |
| 5 mM | 0.31 mL | 1.54 mL | 3.08 mL |
| 10 mM | 0.15 mL | 0.77 mL | 1.54 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.31 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 2017 PMID: 28869757
Product Specific Notices for Janelia Fluor® 585, NHS ester
For research use only
Loading...
Loading...