Janelia Fluor® 552, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 8947
Cell-permeable fluorogenic dye supplier with NHS ester for amine conjugation. Application: live-cell imaging. Also chemigenetic Ca2+ indicator
Key Product Details
Description
Cell-permeable fluorogenic dye supplier with NHS ester for amine conjugation. Application: live-cell imaging. Also chemigenetic Ca2+ indicator
Product Description
Key Information: Janelia Fluor® 552, NHS ester is a yellow cell-permeable fluorogenic dye, supplied with an NHS ester reactive group for the labeling of primary amines. Also chemigenetic calcium indicator for imaging Ca2+ mediated responses. Upon self-labeling in WHaloCaMP modular biosensors, fluorescence output is modulated by photoinduced electron transfer (PET) to a protein based quencher.Application: NHS ester can be converted to relevant substrate for use in self-labeling tag systems. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Also In vivo functional imaging of Ca2+ responses (flies/mice); multiplexed functional imaging of neurons and astrocytes in zebrafish larvae; quantification of Ca2+ concentration using fluorescence lifetime imaging microscopy (FLIM).
Properties and Photophysical Data: Stable to fixation. Cell permeable. Excitation and emission maxima (λ) are 552 nm and 575 nm, respectively; quantum yield = 0.83; extinction coefficient = 129,000 M-1cm-1, KL-Z = 0.70.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| λabs | 552 nm |
| λem | 575 nm |
| Extinction Coefficient (ε) | 129000 M-1cm-1 |
| Quantum Yield (φ) | 0.83 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM), WHaloCaMP |
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Product Specifications
Formula
C31H23F2N3O7
Storage
Store at -20°C
Purity
≥85% (HPLC)
Chemical Name
2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
InChI Key
SWVHORPSJTWCER-UHFFFAOYSA-N
SMILES
FC1=CC2=C(C(C=C3F)=C(OC2=C/C1=[N+]4CCC\4)C=C3N5CCC5)C6=CC(C(ON7C(CCC7=O)=O)=O)=CC=C6C([O-])=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 5.88mg/ml in DMSO |
Calculators
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Product Specific Notices for Janelia Fluor® 552, NHS ester
For research use only
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