Indocyanine green
Tocris Bioscience, part of Bio-Techne | Catalog # 7510
Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging
Key Product Details
Description
Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging
Alternative Names
ICG
Product Description
Key information: Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye suitable for in vivo imaging.Application: Fluorescence microscopy, in vivo imaging.
Properties and Photophysical Data: Indocyanine green is a NIR fluorescent dye operating in the 700-900 nm wavelength range. It binds to β-lipoproteins, particularly albumin, in plasma and exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR. Excitation and emission maxima (λ) are 787 nm and 815 nm, respectively; quantum yield = 0.14; extinction coefficient = 223,000 M-1cm-1; A280 correction factor = 0.07.
This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Optical Data
| Emission Color | Near-IR |
| Brightness | |
| λabs | 787 nm |
| λem | 815 nm |
| Extinction Coefficient (ε) | 223000 M-1cm-1 |
| Quantum Yield (φ) | 0.14 |
| Closest Laser line | 785 nm |
| pH Sensitivity | No |
| Correction Factor 280 | 0.07 |
| Application | Fluorescence Microscopy, in vivo Imaging |
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Product Specifications
Molecular Weight
774.97
Formula
C43H47N2O6S2Na
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
Sodium 4-(2-((1E,3E,5E,7Z)-7-(1,1-Dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate
CAS Number
3599-32-4
PubChem ID
45101199
InChI Key
MOFVSTNWEDAEEK-UHFFFAOYSA-M
SMILES
CC1(/C(N(C2=C1C3=CC=CC=C3C=C2)CCCCS(=O)(O[Na])=O)=C/C=C/C=C/C=C/C4=[N+](C5=C(C4(C)C)C6=CC=CC=C6C=C5)CCCCS([O-])(=O)=O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 38.75 | 50 | |
| DMSO | 15.5 | 20 |
Preparing Stock Solutions
for Indocyanine green
The following data is based on the product molecular weight 774.97
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 2.58 mL | 12.90 mL | 25.81 mL |
| 2.5 mM | 0.52 mL | 2.58 mL | 5.16 mL |
| 5 mM | 0.26 mL | 1.29 mL | 2.58 mL |
| 25 mM | 0.05 mL | 0.26 mL | 0.52 mL |
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Background References
References are publications that support the biological activity of the product.
- Li Recent progress in small-molecule near-IR probes for bioimaging. Trends Chem. 2019 PMID: 32864595
- Lee Surgical outcomes of localization using indocyanine green fluorescence in breast conserving surgery: a prospective study. Sci.Rep. 2021 PMID: 33976314
- Carr Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc.Natl.Acad.Sci.U.S.A. 2018 PMID: 29626132
- Cosco Photophysical properties of indocyanine green in the shortwave infrared region. ChemPhotoChem. 2021 PMID: 34504949
- Starosolski Indocyanine green fluorescence in second near-infrared (NIR-II) window. PLoS One 2017 PMID: 29121078
Product Specific Notices for Indocyanine green
For research use only
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