GF-AFC

Name: GF-AFC
Brand: Tocris Bioscience
SKU: 8143

Tocris Bioscience, part of Bio-Techne | Catalog # 8143

COA / SDS

Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays

Key Product Details

Description:	Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
Chemical Name:	(2S)-2-[(2-Aminoacetyl)amino]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]-3-phenylpropanamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	469.85

View Full Technical Details for GF-AFC

Technical Data
Citations

Biological Activity for GF-AFC

GF-AFC (glycyl-phenylalanyl-aminofluorocoumarin) is a cell permeable peptide fluorogenic substrate. GF-AFC is cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays. GF-AFC can be used as a substrate for measuring the activity of dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease.

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Technical Data for GF-AFC

M. Wt	469.85
Formula	C₂₁H₁₈F₃N₃O₄.HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
InChi Key	GBCCOIYISILSJP-NTISSMGPSA-N
Smiles	NCC(N[C@@H](CC1=CC=CC=C1)C(NC2=CC=C(C(O3)=C2)C(C(F)(F)F)=CC3=O)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GF-AFC

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.98	100

Preparing Stock Solutions for GF-AFC

The following data is based on the product molecular weight 469.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.13 mL	10.64 mL	21.28 mL
5 mM	0.43 mL	2.13 mL	4.26 mL
10 mM	0.21 mL	1.06 mL	2.13 mL
50 mM	0.04 mL	0.21 mL	0.43 mL

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References for GF-AFC

References are publications that support the biological activity of the product.

Niles A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal.Biochem. 2007 PMID: 17512890
Liu Serum based fluorescent assay for evaluating dipeptidyl peptidase I activity in collagen induced arthritis rat model. Mol.Cell.Probes 2017 PMID: 27771442