FITC-labelled Keap1-Nrf2 probe
Tocris Bioscience, part of Bio-Techne | Catalog # 7627
Fluorescent Keap1-Nrf2 peptide
Discontinued Product
7627 has been discontinued.
View all Fluorescent Probes products.
Key Product Details
Description
Fluorescent Keap1-Nrf2 peptide
Product Description
FITC-labelled Keap1-Nrf2 probe is a fluorescent Keap1-Nrf2 peptide (Kd = 22.3 nM). It can be used to assess the binding affinities of Nrf2 peptide inhibitors in Fluorescence Polarization (FP) displacement assays and TR-FRET.Optical Data
| Emission Color | Green |
| λabs | 495 nm |
| λem | 525 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence polarization (FP) displacement assays, TR-FRET |
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Product Specifications
Molecular Weight
1440.50
Formula
C67H81N11O23S
Storage
Store at -20°C
Purity
≥90% (HPLC)
InChI Key
RDECLUWFJCNNAY-NZNAQQBOSA-N
SMILES
O=C(N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H]([C@H](O)C)C(NCC(N[C@@H](CCC(O)=O)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC(C)C)C(N)=O)=O)=O)=O)=O)=O)=O)=O)[C@@H](NC(NC(C=C2C(O)=O)=CC=C2C(C3=C4C=C(O)C=C3)=C(C=C5)C(O4)=CC5=O)=S)CC(C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 2mg/ml in PBS |
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Background References
References are publications that support the biological activity of the product.
- Inoyama Optimization of fluorescently labeled Nrf2 peptide probes and the development of a fluorescence polarization assay for the discovery of inhibitors of Keap1-Nrf2 interaction. J.Biomol.Screen 2012 PMID: 22156223
- Wang Development of an enzyme-linked immunosorbent assay for Keap1-Nrf2 interaction inhibitors identification. Redox Biol. 2020 PMID: 32422542
- Chen Kinetic analyses of Keap1-Nrf2 interaction and determination of the minimal Nrf2 peptide sequence required for Keap1 binding using surface plasmon resonance. Chem.Biol.Drug Des. 2011 PMID: 21920027
Product Specific Notices for FITC-labelled Keap1-Nrf2 probe
For research use only
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