FFN 270

Name: FFN 270
Brand: Tocris Bioscience
SKU: 6717

Tocris Bioscience, part of Bio-Techne | Catalog # 6717

COA / SDS

Fluorescent substrate for NET and VMAT2

Key Product Details

Description:	Fluorescent substrate for NET and VMAT2
Chemical Name:	3-(2-Aminoethyl)-8-fluoro-7-hydroxy-2H-chromen-2-one hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	259.66

View Full Technical Details for FFN 270

Technical Data
Citations

Biological Activity for FFN 270

FFN 270 is a fluorescent false neurotransmitter (FFN). Fluorescent substrate for NET and VMAT2. Labels noradrenergic neurons and their synaptic vesicles, and enables imaging of synaptic vesicle content release from specific axonal sites in vivo. Selectively labels NA neurons over other monoamine and CNS targets. Exhibits two resolved absorption/excitation maxima depending on solvent pH (excitation maxima: 320 nm or 365 nm, emission maxima: 475 nm).

Licensing Information

Sold with the permission of Columbia University.

Scientific Data Examples for FFN 270

	Amphetamine-stimulated release of FFN 270 from noradrenergic projections. Amphetamine releases FFN 270 from noradrenergic projections in vivo. Images show FFN 270 puncta in Layer 1 of the barrel cortex in mice taken before (left) and after injection (right) of amphetamine (AMPH).

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Technical Data for FFN 270

M. Wt	259.66
Formula	C₁₁H₁₀FNO₃.HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2341841-05-0
Smiles	OC1=CC=C2C(OC(C(CCN)=C2)=O)=C1F.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FFN 270

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.97	100

Preparing Stock Solutions for FFN 270

The following data is based on the product molecular weight 259.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.85 mL	19.26 mL	38.51 mL
5 mM	0.77 mL	3.85 mL	7.70 mL
10 mM	0.39 mL	1.93 mL	3.85 mL
50 mM	0.08 mL	0.39 mL	0.77 mL

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References for FFN 270

References are publications that support the biological activity of the product.

Dunn Designing a norepinephrine optical tracer for imaging individual noradrenergic synapses and their activity in vivo. Nat.Commun. 2018 PMID: 30026491