Di 4 ANEPPS
Tocris Bioscience, part of Bio-Techne | Catalog # 7324
Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Key Product Details
Description
Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Product Description
Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.
It is recommended to prepare stock solutions in DMSO.
Optical Data
| Emission Color | Red |
| λabs | 465 nm |
| λem | 635 nm |
| Application | Electrophysiology, Fluorescence Microscopy |
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Product Specifications
Molecular Weight
480.67
Formula
C28H36N2O3S
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate
CAS Number
90134-00-2
PubChem ID
6438327
InChI Key
HAPJROQJVSPKCJ-UHFFFAOYSA-N
SMILES
[O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Tominga & Tominga A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity. J.Neurophysiol. 2013 PMID: 23615547
- Asamoah A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 2003 PMID: 12526775
- Loew A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 1992 PMID: 1469705
Product Specific Notices for Di 4 ANEPPS
For research use only
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