DFHO
Tocris Bioscience, part of Bio-Techne | Catalog # 6434
RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Key Product Details
Description
RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Product Description
DFHO is a mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. DFHO fluorescence is activated by binding to Corn aptamer (Kd = 70 nM) or Squash aptamer (Kd = 54 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M-1 cm-1.Spectra Viewer
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Product Specifications
Molecular Weight
281.22
Formula
C12H9F2N3O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
CAS Number
1420815-34-4
SMILES
CN1C(/C=N/O)=N/C(C1=O)=C\C2=CC(F)=C(C(F)=C2)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.12 | 100 |
Preparing Stock Solutions
for DFHO
The following data is based on the product molecular weight 281.22
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.56 mL | 17.78 mL | 35.56 mL |
| 5 mM | 0.71 mL | 3.56 mL | 7.11 mL |
| 10 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 50 mM | 0.07 mL | 0.36 mL | 0.71 mL |
Calculators
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Dilution Calculator
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Background References
References are publications that support the biological activity of the product.
- Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
- Warner A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 2017 PMID: 28945234
- Dey Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 2022 PMID: 34937909
- Song Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 2017 PMID: 28945233
Product Specific Notices for DFHO
For research use only
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