DA ZP1
Tocris Bioscience, part of Bio-Techne | Catalog # 7444
Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells
Key Product Details
Description
Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells
Product Description
DA ZP1 (diacetylated Zinpyr1) is a fluorogenic Zn(II) sensor (Kd Zn2+ = 0.6 nM). It can be used to image and isolate pancreatic β-cells and β-like cells in vitro. Used to purify live stem cell-derived β-like cells. It can image transplanted islet grafts and endogenous mouse islets in vivo. DA ZP1 is non-fluorescent in the absence of Zn(II) ions but its binding with Zn(II) promotes hydrolytic cleavage of the acetyl groups, generating a strong fluorescent signal. Excitation and emission maxima (λ) are 490 nm and 522 nm, respectively.Optical Data
| λabs | 490 nm |
| λem | 522 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry |
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Product Specifications
Molecular Weight
907.81
Formula
C50H40Cl2N6O7
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
3',6'-bis(Acetyloxy)-4',5'-bis[[bis(2-pyridinylmethyl)amino]methyl]-2',7'-dichlorospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
1816254-69-9
PubChem ID
86305621
InChI Key
HPUUCJUOCSGDIK-UHFFFAOYSA-N
SMILES
CC(OC1=C(C=C2C(OC3=C(C(OC(C)=O)=C(C=C3C24OC(C5=CC=CC=C45)=O)Cl)CN(CC6=CC=CC=N6)CC7=CC=CC=N7)=C1CN(CC8=CC=CC=N8)CC9=CC=CC=N9)Cl)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.82 | 2 |
Preparing Stock Solutions
for DA ZP1
The following data is based on the product molecular weight 907.81
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.02 mM | 55.08 mL | 275.39 mL | 550.78 mL |
| 0.1 mM | 11.02 mL | 55.08 mL | 110.16 mL |
| 0.2 mM | 5.51 mL | 27.54 mL | 55.08 mL |
| 1 mM | 1.10 mL | 5.51 mL | 11.02 mL |
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Background References
References are publications that support the biological activity of the product.
- Zastrow Reaction-based probes for imaging mobile zinc in live cells and tissues. ACS Sens. 2016 PMID: 26878065
- Kahraman Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells. Life Sci.Alliance 2021 PMID: 33514654
Product Specific Notices for DA ZP1
For research use only
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