DA ZP1

Name: DA ZP1
Brand: Tocris Bioscience
SKU: 7444

Tocris Bioscience, part of Bio-Techne | Catalog # 7444

COA / SDS

Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells

Key Product Details

Description:	Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells
Chemical Name:	3',6'-bis(Acetyloxy)-4',5'-bis[[bis(2-pyridinylmethyl)amino]methyl]-2',7'-dichlorospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity:	≥90% (HPLC)
Molecular Weight:	907.81

View Full Technical Details for DA ZP1

Technical Data
Citations

Biological Activity for DA ZP1

DA ZP1 (diacetylated Zinpyr1) is a fluorogenic Zn(II) sensor (K_d Zn²⁺ = 0.6 nM). It can be used to image and isolate pancreatic β-cells and β-like cells in vitro. Used to purify live stem cell-derived β-like cells. It can image transplanted islet grafts and endogenous mouse islets in vivo. DA ZP1 is non-fluorescent in the absence of Zn(II) ions but its binding with Zn(II) promotes hydrolytic cleavage of the acetyl groups, generating a strong fluorescent signal. Excitation and emission maxima (λ) are 490 nm and 522 nm, respectively.

Optical Data for DA ZP1

λ_abs	490 nm
λ_em	522 nm
Cell Permeable	Yes
Application	Flow Cytometry

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Technical Data for DA ZP1

M. Wt	907.81
Formula	C₅₀H₄₀Cl₂N₆O₇
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1816254-69-9
PubChem ID	86305621
InChi Key	HPUUCJUOCSGDIK-UHFFFAOYSA-N
Smiles	CC(OC1=C(C=C2C(OC3=C(C(OC(C)=O)=C(C=C3C24OC(C5=CC=CC=C45)=O)Cl)CN(CC6=CC=CC=N6)CC7=CC=CC=N7)=C1CN(CC8=CC=CC=N8)CC9=CC=CC=N9)Cl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DA ZP1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	1.82	2

Preparing Stock Solutions for DA ZP1

The following data is based on the product molecular weight 907.81.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.02 mM	55.08 mL	275.39 mL	550.78 mL
0.1 mM	11.02 mL	55.08 mL	110.16 mL
0.2 mM	5.51 mL	27.54 mL	55.08 mL
1 mM	1.10 mL	5.51 mL	11.02 mL

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References for DA ZP1

References are publications that support the biological activity of the product.

Zastrow Reaction-based probes for imaging mobile zinc in live cells and tissues. ACS Sens. 2016 PMID: 26878065
Kahraman Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells. Life Sci.Alliance 2021 PMID: 33514654