Cyanine 3 Tyramide
Tocris Bioscience, part of Bio-Techne | Catalog # 6457
Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Key Product Details
Description
Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Product Description
Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data
| Emission Color | Orange |
| λabs | 550 nm |
| λem | 563 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
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Product Specifications
Molecular Weight
749.94
Formula
C39H47N3O8S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
CAS Number
174961-75-2
InChI Key
JPXIOSIRIWBDIC-UHFFFAOYSA-N
SMILES
CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12 | 16 |
Preparing Stock Solutions
for Cyanine 3 Tyramide
The following data is based on the product molecular weight 749.94
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.16 mM | 8.33 mL | 41.67 mL | 83.34 mL |
| 0.8 mM | 1.67 mL | 8.33 mL | 16.67 mL |
| 1.6 mM | 0.83 mL | 4.17 mL | 8.33 mL |
| 8 mM | 0.17 mL | 0.83 mL | 1.67 mL |
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Background References
References are publications that support the biological activity of the product.
- Zhao Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 2013 PMID: 23967349
- Schriml Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 1999 PMID: 10489619
- van Gijlswijk Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 1997 PMID: 9071319
- Hopman Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 1998 PMID: 9603790
- Schmidt Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 1997 PMID: 9071318
Product Specific Notices for Cyanine 3 Tyramide
For research use only
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