cis-Ned 19
Tocris Bioscience, part of Bio-Techne | Catalog # 3953
NAADP antagonist; inhibits Ca2+ release
Discontinued Product
3953 has been discontinued.
View all Calcium Signaling Agents products.
Key Product Details
Description
NAADP antagonist; inhibits Ca2+ release
Product Description
cis-Ned 19 is an irreversible nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist; inhibits Ca2+ release (IC50 = 800 nM) and [32P]NAADP binding (IC50 = 15 μM). Stereoisomer of trans-Ned 19. Fluorescently labels NAADP receptors in intact cells (excitation 351 and 365 nm using an ultraviolet Argon laser).Isomer of trans-Ned 19.
Optical Data
| λabs | 368 nm |
| λem | 425 nm |
| Closest Laser line | 355 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
514.59
Formula
C30H31FN4O3
Storage
Store at -20°C
Purity
≥97% (HPLC)
Chemical Name
(1S,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
CAS Number
1137264-00-6
PubChem ID
1427626
InChI Key
FUHCEERDBRGPQZ-LSYYVWMOSA-N
SMILES
FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H]([C@@](O)=O)N3)=CC=C2OC
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 51.46 | 100 |
Preparing Stock Solutions
for cis-Ned 19
The following data is based on the product molecular weight 514.59
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.72 mL | 19.43 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.89 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Naylor Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 2009 PMID: 19234453
Product Specific Notices for cis-Ned 19
For research use only
Loading...
Loading...