C-Laurdan
Tocris Bioscience, part of Bio-Techne | Catalog # 7273
Two-photon polarity-sensitive lipid membrane probe. Used for: cell membrane imaging. Application: One and Two-photon microscopy for membrane Polarity
Key Product Details
Description
Two-photon polarity-sensitive lipid membrane probe. Used for: cell membrane imaging. Application: One and Two-photon microscopy for membrane Polarity
Product Description
Key information: C-Laurdan is a polarity-sensitive lipid membrane probe.Used for: lipid raft imaging and cell membrane imaging.
Application: One and two-photon microscopy for membrane polarity.
Properties and Photophysical Data: C-laurdan carry a carboxylic group which can be partially ionized which contributes to its good water solubility and fast membrane incorporation. Two photon excitation spectra = 780 nm. One photon excitation and emission maxima (λ) are 348 nm and 423 nm, respectively; quantum yield = 0.43; extinction coefficient = 12,200 M-1cm-1.
Optical Data
| Emission Color | Blue |
| λabs | 348 nm |
| λem | 423 nm |
| Extinction Coefficient (ε) | 12200 M-1cm-1 |
| Quantum Yield (φ) | 0.43 |
| Application | One and Two-photon Microscopy for Membrane Polarity |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
397.56
Formula
C25H35NO3
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-Methyl-N-[6-(1-oxododecyl)-2-naphthalenyl]glycine
CAS Number
959839-06-6
PubChem ID
24966947
InChI Key
INSWREUMXDGFSD-UHFFFAOYSA-N
SMILES
CCCCCCCCCCCC(C1=CC2=C(C=C(N(CC(O)=O)C)C=C2)C=C1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 39.76 | 100 | |
| DMSO | 7.95 | 20 | |
| Ethanol | 3.98 | 10 |
Preparing Stock Solutions
for C-Laurdan
The following data is based on the product molecular weight 397.56
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.52 mL | 12.58 mL | 25.15 mL |
| 5 mM | 0.50 mL | 2.52 mL | 5.03 mL |
| 10 mM | 0.25 mL | 1.26 mL | 2.52 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.50 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Barucha-Kraszewska Will C-Laurdan dethrone Laurdan in fluorescent solvent relaxation techniques for lipid membrane studies? Langmuir 2013 PMID: 23311388
- Mazeres Characterization of M-laurdan, a versatile probe to explore order in lipid membranes. F1000Res. 2014 PMID: 25485094
- Kim A two-photon fluorescent probe for lipid raft imaging: C-laurdan. Chembiochem 2007 PMID: 17300111
Product Specific Notices for C-Laurdan
For research use only
Loading...
Loading...