BL₆₆₀-NO

Name: BL 660 -NO
Brand: Tocris Bioscience
SKU: 7753

Tocris Bioscience, part of Bio-Techne | Catalog # 7753

COA / SDS

Nitric oxide activity-based sensing NIR bioluminescent probe

Key Product Details

Description:	Nitric oxide activity-based sensing NIR bioluminescent probe
Chemical Name:	N-(2-Aminophenyl)-2-((1E,3E)-4-(4-(diethylamino)phenyl)buta-1,3-dien-1-yl)-4,5-dihydrothiazole-4-carboxamide
Purity:	≥90% (HPLC)
Molecular Weight:	420.58

View Full Technical Details for BL₆₆₀-NO

Technical Data
Citations

Biological Activity for BL₆₆₀-NO

BL₆₆₀-NO is an activity-based sensing (ABS) bioluminescent probe for near-infrared red (NIR) detection of nitric oxide (NO). BL₆₆₀-NO is suitable for in vitro, live cell and deep tissue imaging. Used to visualize endogenous NO in vitro, and in murine models of liver and breast cancer. Used to investigate the effect of diet on tumor microenvironment (TME) in mice.

This product is a scalemic mixture of (S) and (R) enantiomers in which (S) is predominant. The exact ratio is batch specific and is expressed as an enantiomeric excess (e.e.) on the Certificate of Analysis.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for BL₆₆₀-NO

M. Wt	420.58
Formula	C₂₄H₂₈N₄OS
Storage	Store at -20°C
Purity	≥90% (HPLC)
InChi Key	LERSAEPSTKIDTF-PIHCAMFYSA-N
Smiles	O=C(NC1=C(N)C=CC=C1)C(CS2)N=C2/C=C/C=C/C(C=C3)=CC=C3N(CC)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BL₆₆₀-NO

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.06	100

Preparing Stock Solutions for BL₆₆₀-NO

The following data is based on the product molecular weight 420.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.38 mL	11.89 mL	23.78 mL
5 mM	0.48 mL	2.38 mL	4.76 mL
10 mM	0.24 mL	1.19 mL	2.38 mL
50 mM	0.05 mL	0.24 mL	0.48 mL

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References for BL₆₆₀-NO

References are publications that support the biological activity of the product.

Yadav Activity-based NIR bioluminescence probe enables discovery of diet-induced modulation of the tumor microenvironment via nitric oxide. ACS Cent.Sci. 2022 PMID: 35505872

BL₆₆₀-NO

Tocris Bioscience, part of Bio-Techne | Catalog # 7753

Key Product Details

Biological Activity for BL₆₆₀-NO

Plan Your Experiments

Technical Data for BL₆₆₀-NO

Solubility for BL₆₆₀-NO

Preparing Stock Solutions for BL₆₆₀-NO

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for BL₆₆₀-NO

Product Documents for BL₆₆₀-NO

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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BL660-NO

Tocris Bioscience, part of Bio-Techne | Catalog # 7753

Key Product Details

Biological Activity for BL660-NO

Plan Your Experiments

Technical Data for BL660-NO

Solubility for BL660-NO

Preparing Stock Solutions for BL660-NO

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for BL660-NO

Product Documents for BL660-NO

COA

Certificate of Analysis

SDS

BL₆₆₀-NO

Biological Activity for BL₆₆₀-NO

Technical Data for BL₆₆₀-NO

Solubility for BL₆₆₀-NO

Preparing Stock Solutions for BL₆₆₀-NO

References for BL₆₆₀-NO

Product Documents for BL₆₆₀-NO