BI 2852 FL
Tocris Bioscience, part of Bio-Techne | Catalog # 8110
Pan RAS Tracer
Key Product Details
Description
Pan RAS Tracer
Product Description
BI 2852 FL is a cell-permeable pan-RAS bioluminescence resonance energy transfer (BRET) fluorescent probe. BI 2852 FL is synthesized by conjugating a fluorophore to a derivative of the reversible SI/II-P KRAS inhibitor, BI 2852. BI 2852 FL is used in competitive BRET format to quantify SII-P engagement to multimeric RAS complexes in live cells.Abs/Em spectra below measured in Ethanol + 0.1% TFA
Optical Data
| λabs | 580 nm |
| λem | 590 nm |
| Extinction Coefficient (ε) | 84,200 M-1cm-1 |
Spectra Viewer
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Product Specifications
Molecular Weight
1088.04
Formula
C59H64BF2N11O7
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-(15-(5,5-Difluoro-7-(1H-pyrrol-2-yl)-5H-5λ4,6λ4-dipyrrolo[1,3,2]diazaborinin-3-yl)-13-oxo-3,6,9-trioxa-12-azapentadecyl)-5-(2-((6-((((3-(6-hydroxy-3-oxoisoindolin-1-yl)-1H-indol-2-yl)methyl)amino)methyl)-1H-indol-1-yl)methyl)-1H-imidazol-1-yl)pentanamide
CAS Number
2762758-95-0
PubChem ID
162640683
InChI Key
VINGSJQDAYACET-UHFFFAOYSA-N
SMILES
O=C1NC(C2=CC(O)=CC=C12)C3=C(NC4=C3C=CC=C4)CNCC5=CC6=C(C=C5)C=CN6CC7=NC=CN7CCCCC(NCCOCCOCCOCCNC(CCC8=CC=C9C=C%10C=CC(C%11=CC=CN%11)=[N+]%10[B-](N98)(F)F)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 10.88 | 10 |
Preparing Stock Solutions
for BI 2852 FL
The following data is based on the product molecular weight 1088.04
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.19 mL | 45.95 mL | 91.91 mL |
| 0.5 mM | 1.84 mL | 9.19 mL | 18.38 mL |
| 1 mM | 0.92 mL | 4.60 mL | 9.19 mL |
| 5 mM | 0.18 mL | 0.92 mL | 1.84 mL |
Calculators
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Dilution Calculator
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Background References
References are publications that support the biological activity of the product.
- Vasta KRAS is vulnerable to reversible switch-II pocket engagement in cells. Nat.Chem.Biol. 2022 PMID: 35314814
Product Specific Notices for BI 2852 FL
For research use only
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