Acridonylalanine

Name: Acridonylalanine
Brand: Tocris Bioscience
SKU: 8086

Tocris Bioscience, part of Bio-Techne | Catalog # 8086

COA / SDS

Fluorescent unnatural amino acid.

Key Product Details

Description:	Fluorescent unnatural amino acid.
Chemical Name:	Sodium 2-Amino-3-(9-oxo-9,10-dihydroacridin-2-yl)propanoate
Purity:	≥95% (HPLC)
Molecular Weight:	304.28

View Full Technical Details for Acridonylalanine

Technical Data
Citations

Biological Activity for Acridonylalanine

Key information: Acridonylalanine is a fluorescent unnatural amino acid. Can be used to specifically label a protein of interest during protein translation. Suitable for lifetime and fluorescence resonance energy transfer (FRET) studies.

Used for: labeling proteins, monitoring protein interactions and conformational changes through fluorescence polarization or lifetime experiments.

Application: fluorescence lifetime imaging microscopy (FLIM), FRET interactions with common fluorophores such as methoxycoumarin.

Properties and Photophysical Data: highly photostable, high quantum yield and long fluorescence lifetime in water. Excitation and emission maxima (λ) are 385 nm and 420 nm, respectively.

Optical Data for Acridonylalanine

λ_abs	385 nm
λ_em	420 nm

Scientific Data Examples for Acridonylalanine

	Source material comparison. Left three bars show the fluorescence intensity of Acridonylalanine (Acd) in GFP protein (incorporated by genetic code expansion at position 151). The source of Acd material originates from either the laboratories of Professor E. James Peterson (EJP) or of Tocris / Bio-Techne (BT). Results are an average of 3 protein expressions in E. coli. The protein without Acd incorporation is included as a negative control. Right three bars: WT GFP expression to determine whether there are any growth effects from Acd in the presence of EJP or BT synthesized Acd.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Acridonylalanine

M. Wt	304.28
Formula	C₁₆H₁₃N₂NaO₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
PubChem ID	496123694
InChi Key	XZLOZGUCQWEABV-UHFFFAOYSA-M
Smiles	O=C1C2=CC(CC(C([O-])=O)N)=CC=C2NC3=CC=CC=C31.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Acridonylalanine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	3.04	10
	water	3.04	10

Preparing Stock Solutions for Acridonylalanine

The following data is based on the product molecular weight 304.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	32.86 mL	164.32 mL	328.64 mL
0.5 mM	6.57 mL	32.86 mL	65.73 mL
1 mM	3.29 mL	16.43 mL	32.86 mL
5 mM	0.66 mL	3.29 mL	6.57 mL

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References for Acridonylalanine

References are publications that support the biological activity of the product.

Speight Efficient synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and Förster resonance energy transfer/luminescence resonance energy transfer studies. J.Am.Chem.Soc. 2013 PMID: 24303933
Hostetler Systematic evaluation of soluble protein expression using a fluorescent unnatural amino acid reveals no reliable predictors of tolerability. ACS Chem.Biol. 2018 PMID: 30216041