Acridine Orange hydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 5092
Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
Key Product Details
Description
Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
Product Description
Key information: Acridine Orange hydrochloride is a nucleic acid binding dye. Cell and organelle permeable.Used for: cell cycle and apoptosis determination, for visual detection of nucleic acids on agarose and polyacrylamide gels, for autophagy analyze.
Application: suitable for flow cytometry and fluorescence microscopy.
Properties and Photophysical Data: emits green fluorescence when incorporated into double-stranded DNA, red fluorescence when bound electrostatically to phosphate groups of RNA or single-stranded DNA, and orange fluorescence in acidic conditions. Excitation and emission maxima (λ) are 502 nm and 525 nm, respectively (green, double strands)/ 460 nm and 650 nm, respectively (red, single strands)/ and 475 nm and 590 nm, respectively (orange, acidic conditions).
This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
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Product Specifications
Formula
C17H19N3.HCl
Storage
Store at RT
Purity
≥98% (HPLC)
Chemical Name
N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
PubChem ID
517204
InChI Key
VSTHNGLPHBTRMB-UHFFFAOYSA-N
SMILES
CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 10mg/ml in water |
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Background References
References are publications that support the biological activity of the product.
- Sato Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange. Sci.Rep. 2018 PMID: 30390005
- McMaster Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 1977 PMID: 73185
- Ratan Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 2008 PMID: 7903353
- Kiyoshima Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 2013 PMID: 24278362
Product Specific Notices for Acridine Orange hydrochloride
For research use only
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