3-Azido-7-hydroxycoumarin
Tocris Bioscience, part of Bio-Techne | Catalog # 7664
Click-reactive fluorescent dye. Suitable for live cell imaging
Key Product Details
Description
Click-reactive fluorescent dye. Suitable for live cell imaging
Product Description
Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.
Optical Data
| Emission Color | Blue |
| λabs | 404 nm |
| λem | 477 nm |
| Application | Fluorescence Microscopy |
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Product Specifications
Molecular Weight
203.15
Formula
C9H5N3O3
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3-Azido-7-hydroxy-2H-1-benzopyran-2-one
CAS Number
817638-68-9
PubChem ID
25127608
InChI Key
KKJNQKVCZCNJDI-UHFFFAOYSA-N
SMILES
[N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 20.32 | 100 | |
| DMSO | 20.32 | 100 |
Preparing Stock Solutions
for 3-Azido-7-hydroxycoumarin
The following data is based on the product molecular weight 203.15
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.92 mL | 24.61 mL | 49.22 mL |
| 5 mM | 0.98 mL | 4.92 mL | 9.84 mL |
| 10 mM | 0.49 mL | 2.46 mL | 4.92 mL |
| 50 mM | 0.10 mL | 0.49 mL | 0.98 mL |
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Background References
References are publications that support the biological activity of the product.
- Washington-Hughes In vivo virus-based macrofluorogenic probes target azide-labeled surface glycans in MCF-7 breast cancer cells. Mol.Pharm. 2013 PMID: 22998503
- Silini CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine. Stem Cell Res.Ther. 2021 PMID: 34641958
- Bobrin Water-borne nanocoating for rapid inactivation of SARS-CoV-2 and other viruses. ACS Nano 2021 PMID: 34423970
- Liu CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne 'click' reaction in water. Nucleic Acids Res 2020 PMID: 32520335
- Li Fluorogenic "click" reaction for labeling and detection of DNA in proliferating cells. Biotechniques 2010 PMID: 20615206
- Sivakumar A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org.Lett. 2004 PMID: 15548086
Product Specific Notices for 3-Azido-7-hydroxycoumarin
For research use only
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