Quantikine Immunoassay Control Set 813 for Human HS PlGF
R&D Systems, part of Bio-Techne | Catalog # QC144
Key Product Details
Species
Applications
Product Summary for Quantikine Immunoassay Control Set 813 for Human HS PlGF
INTENDED USE & DESCRIPTION
For use as quantitative controls for the determination of cytokine concentrations in biological fluids. Concentrations have been assigned using R&D Systems’ Quantikine® kits. Controls are prepared in diluted porcine serum with preservatives. They contain recombinant human cytokines at low, medium and high concentrations. Controls are supplied lyophilized.
STORAGE & STABILITY
Unreconstituted Controls should be stored at 2-8 °C and are stable for at least 6 months from date of receipt. Depending on the analyte of interest, reconstituted controls may be stable when stored at < -20 °C. Users should evaluate the frozen stability of the controls in their application or discard after use.
REAGENT PREPARATION
Reconstitute each vial with the volume of deionized or distilled water indicated on the product datasheet.
PROCEDURE & EXPECTED VALUES
Controls should be assayed in the same manner as unknown specimens.
The acceptable ranges for the analytes in these controls are printed on the product datasheet. Due to possible variations in techniques and methodologies, it is recommended that each laboratory determine its own target range. Laboratories using other test systems should establish their own acceptable ranges as these assays may produce different values.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
• The ranges were determined using R&D Systems’ Quantikine kits. If expected values are not obtained, verify that the lot numbers on the vials correspond with the lot numbers listed above and the correct volume of deionized or distilled water was used for reconstitution of the controls.
• The results obtained with these controls depend upon several factors associated with methods and instrumentation. Test systems other than those supplied by R&D Systems may result in values that differ from those printed on this product datasheet.
Formulation, Preparation, and Storage
Shipping
Storage
Background: PlGF
PlGF (placenta growth factor) is secreted primarily by villous trophoblasts and decidual cells, with circulating levels increasing during pregnancy but attenuated in preeclampsia. PlGF signals through VEGF R1/Flt‑1 and Neuropilins, while VEGF binds both VEGF R1 and R2 but signals mainly through VEGF R2. PlGF reduces binding of VEGF to VEGF R1 and increases VEGF/VEGF R2‑mediated angiogenesis. It induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing and also contribute to inflammation in active sickle cell disease and atherosclerosis.
Long Name
Alternate Names
Gene Symbol
Additional PlGF Products
Product Specific Notices for Quantikine Immunoassay Control Set 813 for Human HS PlGF
For research use only