Mouse Thrombopoietin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MTP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
20 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Product Summary for Mouse Thrombopoietin Quantikine ELISA Kit
The Quantikine Mouse Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure Tpo in cell culture supernates, serum, and plasma. It contains recombinant mouse Tpo and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Tpo showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Tpo.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Tpo
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 123 | 455 | 2364 | 119 | 457 | 2389 |
| Standard Deviation | 9.8 | 17.0 | 58.4 | 9.6 | 21.7 | 115 |
| CV% | 8.0 | 3.7 | 2.5 | 8.1 | 4.7 | 4.8 |
Recovery for Mouse Thrombopoietin Quantikine ELISA Kit
The recovery of mouse Tpo spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 97 | 87-115 |
| Citrate Plasma (n=5) | 104 | 88-113 |
| EDTA Plasma (n=5) | 104 | 87-116 |
| Serum (n=5) | 100 | 88-111 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Tpo in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Thrombopoietin Quantikine ELISA Kit
Mouse Thrombopoietin/Tpo ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thrombopoietin/Tpo
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. Tpo promotes the differentiation, proliferation, and maturation of megakaryocytes and their progenitors. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling.
Alternate Names
MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo
Gene Symbol
THPO
Additional Thrombopoietin/Tpo Products
Product Documents for Mouse Thrombopoietin Quantikine ELISA Kit
Product Specific Notices for Mouse Thrombopoietin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.