Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
R&D Systems, part of Bio-Techne | Catalog # DYC897-2
Discontinued Product
DYC897-2 has been discontinued.
An alternative/replacement product is available:
MCTC0. View all Cytochrome c products.
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Sample Type
Cell Lysates
Reactivity
Mouse, Rat
Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Cytochrome c in cell lysates. An immobilized capture antibody specific for Cytochrome c binds both phosphorylated and unphosphorylated Cytochrome c. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Kit Contents for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cytochrome c
Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
Additional Cytochrome c Products
Product Documents for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
Product Specific Notices for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
For research use only
Loading...
Loading...
Loading...