Mouse/Rat PDGF-BB Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MBB00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
19.3 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat PDGF-BB Quantikine ELISA Kit
The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat PDGF-BB. This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 24 | 24 |
| Mean (pg/mL) | 49.7 | 252 | 823 | 52.1 | 246 | 811 |
| Standard Deviation | 4.5 | 18.7 | 36.7 | 5.3 | 21.1 | 40.4 |
| CV% | 9.1 | 7.4 | 4.5 | 10.2 | 8.6 | 5.0 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 22 | 23 |
| Mean (pg/mL) | 61.1 | 298 | 1019 | 69.5 | 305 | 1048 |
| Standard Deviation | 5.6 | 24.6 | 40.3 | 6.6 | 26.3 | 98.5 |
| CV% | 9.2 | 8.3 | 4.0 | 9.5 | 8.6 | 9.4 |
Recovery for Mouse/Rat PDGF-BB Quantikine ELISA Kit
The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=8) | 100 | 89-118 |
| Mouse Heparin Plasma (n=5) | 96 | 85-113 |
| Rat Cell Culture Supernates (n=8) | 102 | 94-114 |
| Rat EDTA Plasma (n=5) | 102 | 86-111 |
| Rat Heparin Plasma (n=5) | 102 | 85-111 |
| Rat Serum (n=5) | 100 | 89-113 |
Linearity
To assess the linearity of the assay, samples spiked with or containing PDGF-BB in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat PDGF-BB Quantikine ELISA Kit
Mouse/Rat PDGF-BB ELISA Cell Culture Supernate Standard Curve
Mouse/Rat PDGF-BB ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-BB
PDGF-BB (Platelet-Derived Growth Factor BB) is a homodimeric molecule expressed by hepatocytes and nonresorbing osteoclasts, and it promotes osteoblast development and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha/alpha homodimer, alpha/beta heterodimer, and the beta/beta homodimer in vitro, and act through the beta/beta homodimer in vivo.
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
Additional PDGF-BB Products
Product Documents for Mouse/Rat PDGF-BB Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat PDGF-BB Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.