Mouse/Rat PDGF-AB Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MHD00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
3.8 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat PDGF-AB Quantikine ELISA Kit
The Quantikine Mouse/Rat PDGF-AB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-AB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-AB and antibodies raised against recombinant rat PDGF. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-AB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-AB.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat PDGF-AB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Platelet-poor EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 89 | 127 | 89 |
| Mean (pg/mL) | 19 | 58 | 141 | 18 | 60 | 147 |
| Standard Deviation | 1.4 | 2.9 | 4.6 | 1.8 | 3.6 | 7.4 |
| CV% | 7.4 | 5.0 | 3.3 | 10.0 | 6.0 | 5.0 |
Recovery for Mouse/Rat PDGF-AB Quantikine ELISA Kit
The recovery of mouse/rat PDGF-AB spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=6) | 96 | 86-113 |
| Rat Cell Culture Supernates (n=5) | 95 | 80-108 |
| Rat EDTA Plasma (n=6) | 104 | 80-119 |
| Rat Heparin Plasma (n=6) | 98 | 87-109 |
| Rat Serum (n=6) | 97 | 82-112 |
Linearity
To assess the linearity of the assay, samples spiked with or containing PDGF-AB in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse/Rat PDGF-AB Quantikine ELISA Kit
Mouse/Rat PDGF-AB ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AB
PDGF-A and PDGF-B are platelet-derived growth factors that can form disulfide-linked homodimers or heterodimers with each other. These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of PDGF R alpha and R beta. PDGF-AB binds to alpha receptor homodimers as well as the heterodimeric receptor. PDGF-AA and -AB promote cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. They are important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles, and intestinal villus.
Long Name
Platelet-derived Growth Factor AB
Alternate Names
PDGFAB
Additional PDGF-AB Products
Product Documents for Mouse/Rat PDGF-AB Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat PDGF-AB Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.