Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M17AF0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
2.8 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
The Quantikine Mouse IL-17A/F Heterodimer immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-17A/F heterodimer in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-17A/F heterodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-17A/F heterodimer. Results obtained using natural mouse IL-17A/F heterodimer showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17A/F heterodimer.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-17A/F heterodimer
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 22.8 | 119 | 180 | 25.1 | 112 | 188 |
| Standard Deviation | 1.4 | 5.0 | 11.1 | 2.0 | 9.8 | 11.6 |
| CV% | 6.1 | 4.2 | 6.2 | 8.0 | 8.8 | 6.2 |
Recovery for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
The recovery of mouse IL-17A/F heterodimer spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 105 | 93-120 |
| EDTA Plasma (n=4) | 99 | 90-104 |
| Heparin Plasma (n=4) | 91 | 86-98 |
| Serum (n=4) | 91 | 84-98 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of recombinant mouse IL-17A/F heterodimer in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
Mouse IL-17A/F Heterodimer ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17A/F Heterodimer
IL-17A/F is a secreted disulfide-linked heterodimer of the cytokines IL-17A and IL-17F. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells. The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F. IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent). It is upregulated in immune cells during inflammatory arthritis and contributes to disease severity.
Long Name
Interleukin 17 A/F
Alternate Names
CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, Cytotoxic T-Lymphocyte-Associated Protein 8, IL17, IL-17, IL-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A, interleukin-17A
Additional IL-17A/F Heterodimer Products
Product Documents for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
Product Specific Notices for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.