Mouse CXCL4/PF4 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCX400
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)
Sensitivity
0.01 ng/mL
Assay Range
0.078-5 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Product Summary for Mouse CXCL4/PF4 Quantikine ELISA Kit
The Quantikine Mouse PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse PF4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PF4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PF4.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse PF4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.23 | 0.41 | 1.51 | 0.25 | 0.45 | 1.46 |
| Standard Deviation | 0.013 | 0.029 | 0.046 | 0.019 | 0.035 | 0.110 |
| CV% | 5.7 | 7.1 | 3.0 | 7.6 | 7.8 | 7.5 |
Recovery for Mouse CXCL4/PF4 Quantikine ELISA Kit
The recovery of mouse PF4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 102 | 90-120 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse PF4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse CXCL4/PF4 Quantikine ELISA Kit
Mouse CXCL4/PF4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL4/PF4
CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.
Additional CXCL4/PF4 Products
Product Documents for Mouse CXCL4/PF4 Quantikine ELISA Kit
Product Specific Notices for Mouse CXCL4/PF4 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.