Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCX100
Discontinued Product
MCX100 has been discontinued.
View all CXCL10/IP-10/CRG-2 products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.2 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
The Quantikine Mouse IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IP-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IP-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IP-10.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IP-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 106 | 217 | 836 | 107 | 250 | 874 |
| Standard Deviation | 4.2 | 10 | 50 | 11 | 20 | 67 |
| CV% | 4.0 | 4.6 | 6.0 | 10.3 | 8.0 | 7.7 |
Recovery for Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
The recovery of mouse IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 97 | 88-104 |
| Heparin Plasma (n=6) | 109 | 93-118 |
| Serum (n=5) | 97 | 81-118 |
Linearity
To assess the linearity of the assay, four samples containing and/or spiked with high concentrations of mouse IP-10 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
Mouse CXCL10/IP-10/CRG-2 Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
Product Specific Notices for Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
For research use only
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