Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39810
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.29 ng/mL (example only; lot dependent)
Assay Range
0.937 - 60 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Assay Length: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
ELISA: Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39810]
ELISA: Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39810] - Standard Curve ReferenceKit Contents for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
- Detection Solution A
- Detection Solution B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: MEPE/OF45
MEPE, known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family that is primarily expressed in bone and dentin where it regulates the mineralization of those tissues. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process and also by non-mineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif.
Long Name
Matrix Extracellular Phosphoglycoprotein with ASARM Motif
Alternate Names
OF45, Osteoregulin
Gene Symbol
MEPE
Additional MEPE/OF45 Products
Product Documents for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
Product Specific Notices for Human MEPE/OF45 - Ready-To-Use ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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