Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67959
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
37.50 pg/mL
Assay Range
62.50 - 4000 pg/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Rat
Specificity
This kit recognizes Rat FNDC5 in samples. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 4.91%
Inter-Assay Precision (Precision between assays) CV% < 4.88%
Recovery for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
Recovery
87-102%
Linearity
Scientific Data Images for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
ELISA: Rat Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP2-67959] -
ELISA: Rat Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP2-67959] - Standard Curve ReferenceKit Contents for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Irisin/FNDC5
Irisin, also known as FNDC5, is a peptide hormone that is upregulated in skeletal muscle and subcutaneous adipose tissue during exercise. It induces expression of the mitochondrial proteins PPAR-gamma coactivator 1 alpha (PGC1a) and uncoupling protein-1 (UCP1). It contributes to the regulation of diabetes and obesity by inducing the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation, neurite outgrowth, and osteoblast differentiation.
Long Name
Fibronectin Type III Domain Containing 5
Alternate Names
FNDC5, FRCP2
Gene Symbol
FNDC5
Additional Irisin/FNDC5 Products
Product Documents for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
Product Specific Notices for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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