Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

Name: Chicken Insulin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-50074

I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Chicken Insulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50074

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Chicken

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Chicken Insulin ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Scientific Data Images for Chicken Insulin ELISA Kit (Colorimetric)

ELISA: Chicken Insulin ELISA Kit (Colorimetric) [NBP3-50074]

Standard Curve Reference.

Kit Contents for Chicken Insulin ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Certificate of Analysis
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Description
Reference Standard
Reference Standard & Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Chicken Insulin ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Chicken Insulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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