Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49817

Novus Biologicals, part of Bio-Techne
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NBP3-49817
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates, and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all LILRB2/CD85d/ILT4 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes HumanI LT4/CD85d/LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

Recovery

80 - 120%

Linearity

Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

ELISA: Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence) [NBP3-49817]

Standard Curve Reference.

Kit Contents for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate(100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LILRB2/CD85d/ILT4

LILRB2, also known as Leukocyte immunoglobulin-like receptor subfamily B member 2, has 2 isoforms, a 598 amino acid isoform that is 65 kDa and a 597 amino acid isoform that is approx. 65 kDa; expressed on monocytes and B-cells, and at lower levels on dendritic cells and in natural killer (NK) cells; as a receptor binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response; also competes with CD8A for binding to class I MHC antigens and inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Disease research is currently being performed with relation to LILRB2 and herpes simplex, chronic lymphocytic leukemia, lymphocytic leukemia, and leukemia. Interactions with this protein have been shown to involve PTPN6, FCGR1A, HLA-F, HLA-G, CD1D and other proteins in osteoclast differentiation, immune-regulatory interactions between a lymphoid and a non-lymphoid cell, adaptive immune system, and immune system pathways.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5

Alternate Names

CD85d, ILT4, LIR2, MIR10

Gene Symbol

LILRB2

Additional LILRB2/CD85d/ILT4 Products

Product Documents for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ILT4/CD85d/LILRB2 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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