IL-36 gamma/IL-1F9 Antibody Pair

Sandwich ELISA

3.9 - 250 pg/ml

Cell Culture Supernatant, Serum (100 ul)

1 pg/ml

Human

Sandwich ELISA for the quantitative determination of Human IL-36 gamma/IL-1F9

100 ul

Unconjugated

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 2 ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (0.1 mg/ml) (mouse monoclonal antibody) to 0.1 ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant human IL-36 gamma/IL-1F9) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml, 7.8 pg/ml, 3.9 pg/ml and 0 pg/ml.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.