Mouse IL-35 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06750
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 uL)
Sensitivity
9.38 pg/mL
Assay Range
15.63 - 1000 pg/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Mouse
Specificity
This kit recognizes Mouse IL-35 in samples. No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) %CV < 5.14
Inter-Assay Precision (Precision between assays) %CV < 4.64
Recovery for Mouse IL-35 ELISA Kit (Colorimetric)
Recovery
88 - 110%
Linearity
Scientific Data Images for Mouse IL-35 ELISA Kit (Colorimetric)
ELISA: Mouse IL-35 ELISA Kit (Colorimetric) [NBP3-06750] -
ELISA: Mouse IL-35 ELISA Kit (Colorimetric) [NBP3-06750] - Standard Curve ReferenceKit Contents for Mouse IL-35 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL-35
IL-35 (Interleukin 35) is an immunosuppressive IL-12 family heterodimeric cytokine that contains a p35 subunit and the EBI3 subunit. It is secreted by Treg and effector T cells and functions through IL-12 R beta 2 and gp130. IL-35 can suppress effector T cell proliferation, inhibit Th17 cell development, and promote the conversion of T cells and B cells into regulatory T and B cells, respectively. It also functions as a regulator of tumor growth.
Long Name
Interleukin 35
Alternate Names
IL35
Gene Symbol
EBI3
Additional IL-35 Products
Product Documents for Mouse IL-35 ELISA Kit (Colorimetric)
Product Specific Notices for Mouse IL-35 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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