Human VEGFR2/KDR Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DVR200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
11.4 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human VEGFR2/KDR Quantikine ELISA Kit
The Quantikine Human VEGF R2 Immunoassay is a 4.5 hour solid-phase Immunoassay designed to measure human soluble VEGF R2 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human VEGF R2/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human soluble VEGF R2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human VEGF R2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 465 | 1269 | 2995 | 455 | 1233 | 2962 |
| Standard Deviation | 19.4 | 45.5 | 87.9 | 32.1 | 84.8 | 169 |
| CV% | 4.2 | 3.6 | 2.9 | 7.0 | 6.9 | 5.7 |
Recovery for Human VEGFR2/KDR Quantikine ELISA Kit
The recovery of VEGF R2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 92-104 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of VEGF R2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VEGFR2/KDR Quantikine ELISA Kit
Human VEGF R2/KDR/Flk-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGFR2/KDR/Flk-1
VEGFR2/KDR/Flk-1 (vascular endothelial growth factor receptor 2) is a transmembrane receptor tyrosine kinase that mediates the angiogenic effects of VEGF-A and VEGF-C. It is expressed primarily on vascular endothelial cells and endothelial cell progenitors. It is also expressed on endometrial epithelium, hematopoietic stem cells, liver sinusoidal endothelial cells, Sertoli cells and Leydig cells, platelets and megakaryocytes, sensory and autonomic neurons, Schwann cells, Muller glial cells, retinal progenitors, and osteoblasts. VEGFR2 can associate with VEGFR1, and a soluble form of VEGFR2 can circulate in the serum.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Gene Symbol
KDR
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for Human VEGFR2/KDR Quantikine ELISA Kit
Product Specific Notices for Human VEGFR2/KDR Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.