Human Total HO-1/HMOX1 DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC3776-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
156-10000 pg/mL
Sample Type
Cell Lysates
Reactivity
Human
Human Total HO-1/HMOX1 DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Total HO-1/HMOX1 DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total HO-1 / HMOX1 / HSP32 in cell lysates. An immobilized capture antibody specific for HO-1 / HMOX1 / HSP32 binds both phosphorylated and unphosphorylated HO-1 / HMOX1 / HSP32. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Total HO-1/HMOX1 DuoSet IC ELISA
Standard Curve
Kit Contents for Human Total HO-1/HMOX1 DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase (HO), also known as HMOX, is the rate limiting enzyme in the breakdown of heme into Biliverdin, CO, and iron. There are two major isoforms of this enzyme, HO-1 and HO-2. Both have similar enzymatic activity; however, HO-1 is rapidly inducible, while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress, infection, hypoxia, and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active, and the enzyme has a broad range of putative roles. For instance, Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties, and CO has the potential to suppress inflammation and apoptosis. Because of these activities, HO-1 has received much attention as a therapeutic target for a range of pathological processes.
Long Name
Heme Oxygenase 1
Alternate Names
HMOX1, HO1, HSP32
Gene Symbol
HMOX1
Additional HO-1/HMOX1/HSP32 Products
Product Documents for Human Total HO-1/HMOX1 DuoSet IC ELISA
Product Specific Notices for Human Total HO-1/HMOX1 DuoSet IC ELISA
For research use only
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