Human Total ErbB4/Her4 DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC1133-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
125-8000 pg/mL
Sample Type
Cell Lysates
Reactivity
Human
Human Total ErbB4/Her4 DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Total ErbB4/Her4 DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ErbB4 / Her4 in cell lysates. An immobilized capture antibody specific for ErbB4 / Her4 binds both phosphorylated and unphosphorylated ErbB4 / Her4. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Total ErbB4/Her4 DuoSet IC ELISA
Standard Curve
The Human Total ErbB4 DuoSet IC ELISA is more sensitive than Western Blot analysis
Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) Western Blot and (B) this DuoSet IC ELISA. Immunoblots were incubated with an anti-ErbB4 polyclonal antibody to detect total ErbB4. Bands were visualized with anti-rabbit IgG-HRP (Catalog # HAF008) followed by chemiluminescent detection. Human ErbB4 can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western Blot.
The Human Total ErbB4 DuoSet® IC ELISA measures the relative level of ErbB4.
Lysates were prepared from MDA-MB-453 human breast cancer cells and the MCF-7 human breast cancer cell line. Both ELISA and Western Blot (inset) analyses were performed using 100 μg of lysate.Kit Contents for Human Total ErbB4/Her4 DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product insert.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ErbB4/Her4
ErbB4, also known as Her4, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the EGF family of growth factors. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF).
Long Name
Receptor Tyrosine Protein Kinase ErbB4
Alternate Names
HER4
Gene Symbol
ERBB4
Additional ErbB4/Her4 Products
Product Documents for Human Total ErbB4/Her4 DuoSet IC ELISA
Product Specific Notices for Human Total ErbB4/Her4 DuoSet IC ELISA
For research use only
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