Human Thrombospondin-2 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DTSP20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Human Milk (50 µL)
Sensitivity
0.068 ng/mL
Assay Range
0.31-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
Product Summary for Human Thrombospondin-2 Quantikine ELISA Kit
The Quantikine Human Thrombospondin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Thrombospondin-2 in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human Thrombospondin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombospondin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombospondin-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Thrombospondin-2
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 4.08 | 7.73 | 10.9 | 4.11 | 7.71 | 11.4 |
| Standard Deviation | 0.189 | 0.229 | 0.281 | 0.295 | 0.511 | 0.726 |
| CV% | 4.6 | 3.0 | 2.6 | 7.2 | 6.6 | 6.4 |
EDTA Plasma, Heparin Plasma, Human Milk, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.97 | 7.96 | 11.4 | 4.12 | 7.68 | 11.4 |
| Standard Deviation | 0.198 | 0.205 | 0.284 | 0.259 | 0.403 | 0.527 |
| CV% | 5.0 | 2.6 | 2.5 | 6.3 | 5.2 | 4.6 |
Recovery for Human Thrombospondin-2 Quantikine ELISA Kit
The recovery of Thrombospondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 91-108 |
| EDTA Plasma (n=4) | 93 | 86-105 |
| Heparin Plasma (n=4) | 92 | 86-103 |
| Serum (n=4) | 93 | 86-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Thrombospondin-2 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Thrombospondin-2 Quantikine ELISA Kit
Human Thrombospondin-2 ELISA Cell Culture Supernate Standard Curve
Human Thrombospondin-2 ELISA Serum/Plasma/Human Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thrombospondin-2
Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.
Additional Thrombospondin-2 Products
Product Documents for Human Thrombospondin-2 Quantikine ELISA Kit
Product Specific Notices for Human Thrombospondin-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.