Human Thrombospondin-1 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DTSP10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), Platelet-poor EDTA Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL), Saliva (50 µL), Human Milk (50 µL)
Sensitivity
0.944 ng/mL
Assay Range
7.81-500 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk)
Product Summary for Human Thrombospondin-1 Quantikine ELISA Kit
The Quantikine Human Thrombospondin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Thrombospondin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Thrombospondin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombospondin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombospondin-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Thrombospondin-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 71.9 | 142 | 282 | 75.8 | 151 | 294 |
| Standard Deviation | 4.34 | 9.56 | 18.0 | 4.71 | 8.51 | 17.6 |
| CV% | 6.0 | 6.7 | 6.4 | 6.2 | 5.6 | 6.0 |
Recovery for Human Thrombospondin-1 Quantikine ELISA Kit
The recovery of Thrombospondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-101 |
| Platelet-poor EDTA Plasma (n=4) | 94 | 89-99 |
| Platelet-poor Heparin Plasma (n=4) | 93 | 87-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Thrombospondin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thrombospondin-1
Thrombospondin-1 (TSP-1) is a secreted homotrimeric protein that regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling. In the nervous system, it promotes excitatory synapse formation and supports the integration of neural progenitor cells into the olfactory bulb.
Alternate Names
THBS1, Thrombospondin1, TSP-1
Gene Symbol
THBS1
Additional Thrombospondin-1 Products
Product Documents for Human Thrombospondin-1 Quantikine ELISA Kit
Product Specific Notices for Human Thrombospondin-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.