Human TFF3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTFF30
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
Sensitivity
13.1 pg/mL
Assay Range
39-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human TFF3 Quantikine ELISA Kit
The Quantikine Human TFF3 Immunoassay is a 4.5 hour solid-phase
ELISA designed to measure human TFF3 in cell culture supernates,
serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human TFF3 and has been shown to accurately quantitate the recombinant
factor. Results obtained using natural human TFF3 showed linear
curves that were parallel to the standard curves obtained using the
Quantikine kit standards. These results indicate that this kit can be
used to determine relative mass values for naturally occurring human TFF3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Trefoil Factor 3 (TFF3)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean (pg/mL) | 276 | 849 | 1684 | 286 | 894 | 1712 |
| Standard Deviation | 6.01 | 9.13 | 37.4 | 18.3 | 44.0 | 95.9 |
| CV% | 2.2 | 1.1 | 2.2 | 6.4 | 4.9 | 5.6 |
Recovery for Human TFF3 Quantikine ELISA Kit
The recovery of human TFF3 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 100 | 90-114 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human TFF3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TFF3 Quantikine ELISA Kit
Human TFF3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TFF3
TFF3 (Trefoil Factor 3), also known as ITF and P1.B, is a peptide that plays an important role in epithelial regeneration and wound healing. It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2. TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach as well as by chondrocytes during bone development. TFF3 is upregulated in response to gastrointestinal epithelial disruptions and promotes epithelial wound healing. It enhances tumor cell invasion and metastasis by supporting hypoxia-induced VEGF upregulation.
Long Name
Trefoil Factor 3
Alternate Names
ITF, TFI
Gene Symbol
TFF3
Additional TFF3 Products
Product Documents for Human TFF3 Quantikine ELISA Kit
Product Specific Notices for Human TFF3 Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.