Human sTfR Quantikine IVD ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTFR1
This product has been discontinued.
Discontinued Product
DTFR1 has been discontinued.
View all TfR (Transferrin R) products.
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Citrate Plasma (20 µL)
Sensitivity
0.5 nmol/L
Assay Range
3-80 nmol/L (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human sTfR Quantikine IVD ELISA Kit
The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD). For In Vitro Diagnostic Use.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Measures human sTfR levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with other substances.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 60 | 60 | 60 |
| Mean (nmol/L) | 6.9 | 18.2 | 40.2 | 10.9 | 25.7 | 67.2 |
| Standard Deviation | 0.3 | 1.3 | 2.5 | 0.7 | 1.4 | 3.8 |
| CV% | 4.3 | 7.1 | 6.2 | 6.4 | 5.4 | 5.7 |
Recovery for Human sTfR Quantikine IVD ELISA Kit
The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Citrate Plasma (n=5) | 112 | 105-121 |
| EDTA Plasma (n=5) | 103 | 97-112 |
| Heparin Plasma (n=5) | 106 | 101-112 |
| Serum (n=5) | 97 | 95-100 |
Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.
Scientific Data Images for Human sTfR Quantikine IVD ELISA Kit
Human TfR (Transferrin R) In Vitro Diagnostic ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TfR (Transferrin R)
Transferrin Receptor (TfR, TfR-1, CD71) is a transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a disulfide-linked homodimer. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Proteolytic shedding of TfR into the circulation is enhanced by iron deficiency. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR and targets TfR for degradation rather than recycling. Cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. TfR carries out ferritin-independent functions in T cell development, immunological synapse formation and Galectin-3-mediated cell death, and it serves as a cell entry receptor for New World hemorrhagic fever arenaviruses.
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
Additional TfR (Transferrin R) Products
Product Documents for Human sTfR Quantikine IVD ELISA Kit
Product Specific Notices for Human sTfR Quantikine IVD ELISA Kit
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