Human PSG1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPSG10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 ng/mL), Serum (50 ng/mL), EDTA Plasma (50 ng/mL), Heparin Plasma (50 ng/mL)
Sensitivity
0.48 ng/mL
Assay Range
3.13-200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human PSG1 Quantikine ELISA Kit
The Quantikine Human PSG-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PSG-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 18.7 | 59.4 | 118 | 20.0 | 62.4 | 121 |
| Standard Deviation | 0.36 | 0.79 | 1.7 | 1.2 | 2.4 | 4.9 |
| CV% | 1.9 | 1.3 | 1.4 | 6.0 | 3.8 | 4.0 |
Recovery for Human PSG1 Quantikine ELISA Kit
The recovery of PSG-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 83-118 |
| EDTA Plasma (n=4) | 92 | 84-108 |
| Heparin Plasma (n=4) | 90 | 83-99 |
| Serum (n=4) | 91 | 81-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PSG-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PSG1 Quantikine ELISA Kit
Human PSG-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PSG1
Pregnancy-specific beta-1 glycoprotein 1 (PSG1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. PSGs are part of the carcinoembryonic antigen (CEA) family and serve as early biochemical markers of syncytiotrophoblast formation. PSGs, which are produced by syncytial cells in the placenta, are detectable in the maternal plasma as early as 2-3 weeks following fertilization and increase as pregnancy progresses. They are alleged to promote immune tolerance of the semi-allotypic fetus during pregnancy. PSG1 fosters an anti-inflammatory uterine environment by inducing IL-10, IL-6, and TGF-beta 1 secretion. Additionally, PSG1 is believed to mediate placental vascular morphogenesis by enhancing VEGF-A production and endothelial tube formation.
Long Name
Pregnancy-specific beta-1-glycoprotein 1
Alternate Names
B1G1, CD66f, DHFRP2, PBG1, PS-beta-G-1, PSbG1, PSG-1, PSG95, PSGGA
Entrez Gene IDs
5669 (Human)
Gene Symbol
PSG1
Additional PSG1 Products
Product Documents for Human PSG1 Quantikine ELISA Kit
Product Specific Notices for Human PSG1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.